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姓 名
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吳定峰
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職 稱
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教授
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校 內 分 機
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( 06 )-2533131 # 3935
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辦公室
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I棟大樓911室
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E - mail
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wutingfe@
stust.edu.tw
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最 高 學 歷
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密西根州立大學微生物學博士
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研究領域
專長 : 分子生物及蛋白質體學
1. 以蛋白質體學技術尋找膀胱癌的分子符號
2. 以蛋白質體學技術尋找食材或中草藥療效有關基因,如蛹蟲草與紅石榴等
3. 以生物技術方法開發食材或中草藥療效初步篩選平台,如抗過敏與保腎等
經歷
服務機關
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職稱
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起迄年月
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南臺科技大學生物科技系
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系主任
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107年8月-110年7月
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南臺科技大學生物科技系
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教授
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95-至今
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南臺科技大學生物科技系
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副教授
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91-94
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嘉南藥理科技大學藥學系
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副教授
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85-91
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期刊論文(1996-present)
1. Wu
TF*, Shi WY, Chiu YC, Chan YY (2021, Mar.). Investigation of the molecular
mechanism underlying the inhibitory activities of ethanol extract of Bombyx
mori pupa-incubated Cordyceps militaris fruiting bodies toward allergic
rhinitis. Biomedicine and Pharmacotherapy 135, 111248.
2. Shaikh MO, Huang TC, Wu TF, Chuang CH (2020,
June). Label-free Impedimetric Immunosensor for effective bladder Cancer
detection in clinical urine samples. Biomedical Microdevices 22(3), 45.
3. YIN-HSUN FENG,
CHAO-LING TUNG, YU-CHU SU, CHAO-JUNG TSAO, TING-FENG WU (2019, Nov). Proteomic
Profile of Sorafenib Resistance in Hepatocellular Carcinoma; GRP78 Expression
Is Associated With Inferior Response to Sorafenib.. Cancer Genomics &
Proteomics, 16(6), 569-576. (SCI, 109/230, oncology).
4. Shaikh MO, Chang LY,
Chen CH, Wu TF, Chuang CH. (2018, Nov). Paper-based immunosensor utilizing
dielectrophoretic trapping of microprobes for quantitative and label free
detection using electrochemical impedance spectroscopy.. Biomicrofluidics,
12(6), 064102.
5. Chang CP, Chan YY,
Li CF, Chien LH, Lee ST, Wu TF*. (2018). Deciphering the Molecular Mechanism
Underlying the Inhibitory Efficacy of Taiwanese Local Pomegranate Peels against
Urinary Bladder Urothelial Carcinoma. Nutrients. 10: pii: E543.
6. Li CF, Shen KH,
Chien LH, Huang CH, Wu TF*, He HL. (2018). Proteomic Identification of the
Galectin-1-Involved Molecular Pathways in Urinary Bladder Urothelial Carcinoma.
Int J Mol Sci. 19: pii: E1242.
7. Wu TF, Chan YY, Shi
WY, Jhong MT. (2017). Uncovering the Molecular Mechanism of Anti-Allergic
Activity of Silkworm Pupa-Grown Cordyceps militaris Fruit Body. Am J Chin Med.
45(3):497-513.
8. Chang IW, Wang YH,
Wu WJ, Liang PI, Li WM, Yeh BW, Wu TF, He HL, Huang SK, Li CF. (2016). Necdin
Overexpression Predicts Poor Prognosis in Patients with Urothelial Carcinomas
of the Upper Urinary Tract and Urinary Bladder. J. Cancer 7(3): 304-313.
9. Ma LJ, Wu WJ, Wang
YH, Wu TF, Liang PI, Chang IW, He HL, Li CF. (2016). SPOCK1 Overexpression
Confers a Poor Prognosis in Urothelial Carcinoma. J. Cancer 7(4): 467-476.
10. Lee YY, Wu WJ, Huang
CN, Li CC, Li WM, Yeh BW, Liang PI, Wu TF, Li CF. (2016). CSF2 Overexpression
Is Associated with STAT5 Phosphorylation and Poor Prognosis in Patients with
Urothelial Carcinoma. J. Cancer 7(6): 711-721.
11. Wu
TF*, Hsu LT, Tsang BX, Huang LC, Shih WY, Chen LY. (2016). Clarification of the
molecular pathway of Taiwan local pomegranate fruit juice underlying the
inhibition of urinary bladder urothelial carcinoma cell by proteomics strategy.
BMC Complement. Altern. Med. 16(1): 96.
12. Chang
IW, Wu WJ, Wang YH, Wu TF, Liang PI, He HL, Yeh BW, Chien-Feng Li CF. (2016).
BCAT1 overexpression is an indicator of poor prognosis in patients with
urothelial carcinomas of the upper urinary tract and urinary bladder.
Histopathology 68(4): 520-532.
13. Chuang
CH, Wu HP, Huang YW, Chen CH, Lee DH, Wu TF. (2016). A Rapid and Sensitive
Impedance-Based Immunosensor Utilizing Dielectrophoretic Manipulations of
Polyaniline Modified Nanoprobes. IEEE Sensors J. 16(11): 4166-4173.
14. Chuang
CH, Du YC, Wu TF, Chen CH, Lee DH, Chen SM, Huang TC, Wu HP, Shaikh MO. (2016).
Immunosensor for the ultrasensitive and quantitative detection of bladder
cancer in point of care testing. Biosensors and Bioelectronics 84: 126-132.
15. Shen
KH, Li CF, Chien LH, Huang CH, Su CC, Liao AC, Wu TF. (2016). Role of
galectin-1 in urinary bladder urothelial carcinoma cell invasion through the
JNK pathway. Cancer Sci. 2016 Oct;107(10):1390-1398.
16. Su
CC, Sheng KH, Chen SY, Tsay YG, Wu TF. (2016). Searching urinary
tumor-associated proteins for bladder transitional cell carcinoma in
southwestern Taiwan using gel-based proteomics. Urol. Sci. : in press.
17. Wu
HF, Amstislavskaya TG, Chen PH, Wu TF, Chen YH, Jen CP.
Preconcentration-enhanced immunosensing for whole human cancer cell lysate
based on a nanofluidic preconcentrator. BioChip J. 10(3): 159-166.
18. Ting-Feng
Wu, Jyun-Yi Ma and Chien-Tai Cheng (2015). Suppression of upper urinary tract
urothelial carcinoma by the ethanol extract of silkworm pupa cultivated Cordyceps
militaris fruit body through G2/M-phase arrest . IWRIS2015
Proceedings, IWRIS2015, 28-31. (proceedings)..
19. Chang
IW, Liang PI, Li CC, Wu WJ, Huang CN, Lin VC, Hsu CT, He HL, Wu TF, Hung
CH, Li CF (2015). HAS3 underexpression as an indicator of poor prognosis
in patients with urothelial carcinoma of the upper urinary tract
and urinary bladder. Tumor biology, 36(7), 5441-5450.
20. Eric
W. Fan, Ching-Chia Li, Wen-Jeng Wu, Chun-Nung Huang, Wei-Ming
Li, Hung-Lung Ke, Hsin-Chih Yeh, Ting-Feng Wu, Peir-In Liang, Li-Jung Ma
and Chien-Feng Li (2015). FGF7 Over Expression is an
Independent Prognosticator in Patients with Urothelial Carcinoma of the
Upper Urinary Tract
and Bladder. Journal of Urology,
194(1), 223-229.
21. Chuang
CH, Wu TF, Chen CH, Chang KC, Ju JW, Huang YW, Nhana VV (2015). Lab on a chip
for multiplexed immunoassays to detect bladder cancer using multifunctional
dielectrophoretic manipulations. Lab on a chip, 15(14): 3056-3064.
22. He
HL, Lee YE, Chen HP, Hsing CH, Chang IW, Shiue YL, Lee SW, Hsu CT, Lin LC, Wu
TF, Li CF (2015). Overexpression of DNAJC12 predicts poor response to
neoadjuvant concurrent chemoradiotherapy in patients with rectal cancer.
Experimental and molecular pathology, 98(3), 338-345.
23. He
HL, Lee YE, Shiue YL, Lee SW, Lin LC, Chen TJ, Wu TF, Li CF (2015). PLA2G2A
overexpression is associated with poor therapeutic response and inferior
outcome in rectal cancer patients receiving neoadjuvant concurrent
chemoradiotherapy.. Histopathology, 66(7), 991-1002.
24. Chang
IW, Lin VCH, He HL, Hsu CT, Li CC, Wu WJ, Huang CN, Ting-Feng Wu TF, Li CF.
(2015). CDCA5 overexpression is an indicator of poor prognosis in patients with
urothelial carcinomas of the upper urinary tract and urinary bladder. Amer. J.
Trans. Res. 7(4): 710-722.
25. Wu
TF, Li CF, Chien LH, Shen KH, Huang HY, Su CC3, Liao AC (2015). Galectin-1
dysregulation independently predicts disease specific survival in bladder
urothelial carcinoma. J. Urol. 193(3): 1002-1008.
26. Li
CF, He HL, Wang JY, Huang HY, Wu TF, Hsing CH, Lee SW, Lee HH, Fang JL, Huang
WT, Chen SH (2014). Fibroblast growth factor receptor 2 overexpression is
predictive of poor prognosis in rectal cancer patients receiving neoadjuvant
chemoradiotherapy. J. Clin. Pathology, 67(12):1056-1061.
27. He
HL, Lee YE, Shiue YL, Lee SW, Lin LC, Chen TJ, Wu TF, Hsing CH, Huang HY, Wang
JY, Li CF. (2014). Overexpression of REG4 confers an independent negative
prognosticator in rectal cancers receiving concurrent chemoradiotherapy.
Journal of Surgical Oncology, 110(8):1002-1010.
28. Lee
YY, Li CF, Lin CY, Lee SW, Sheu MJ, Lin LC, Chen TJ, Wu TF, Hsing CH (2014).
Overexpression of CPS1 is an independent negative prognosticator in rectal
cancers receiving concurrent chemoradiotherapy. Tumor Biology, 35(11):
11097-11105.
29. Chuang CH,
Yu YC, Lee DH, Wu TF, Chen CH, Chen SM, Wu HP, Huang YW (2014). Miniaturization
of immunoassay by using a novel module-level immunosensor with
polyaniline-modified nanoprobes that incorporate impedance sensing and
paper-based sampling. Microfluidics and Nanofluidics, 16(5): 869-877.
30. Lee
ST, Lu MH, Chien LH, Wu TF*, Huang LC, Liao GI (2013). Suppression of urinary
bladder urothelial carcinoma cell by the ethanol extract of pomegranate fruit
through cell cycle arrest and apoptosis. BMC Complement. Altern. Med., 13(1):
364.
31. Hsieh
CH, Chen GC, Chen PH, Wu TF*, Chao PM*. (2013). Altered White Adipose Tissue
Protein Profile in C57BL/6J Mice Displaying Delipidative, Inflammatory, and
Browning Characteristics after Bitter Melon Seed Oil Treatment. PLoS One,
8(9):e72917.
32. Liang
PI, Wang YH, Wu TF, Wu WR, Liao AC, Shen KH, Hsing CH, Shiue YL, Huang HY, Hsu
HP, Chen LT, Lin CY, Tai C, Wu JY, Li CF. (2013). IGFBP-5 overexpression as a
poor prognostic factor in patients with urothelial carcinomas of upper urinary
tracts and urinary bladder.. Journal of Clinical Pathology 66(7): 573-582.
33. Liang
PI, Li WM, Wang YH, Wu TF, Wu WR, Liao AC, Shen KH, Wei YC, Hsing CH, Shiue YL,
Huang HY, Hsu HP, Chen LT, Lin CY, Tai C, Lin CM, Li CF. (2012). HuR
Cytoplasmic Expression Is Associated with Increased Cyclin A Expression and Poor
Outcome with Upper Urinary Tract Urothelial Carcinoma.. BMC Cancer 12: 611.
34. Lee
ST, Wu YL, Chien LH, Chen ST, Tzeng YK, Wu TF* (2012). Proteomic exploration of
the impacts of pomegranate fruit juice on the global gene expression of
prostate cancer cell. Proteomics 12(21): 3251-3262.
35. Liang
PI, Wu LC, Sheu JJ, Wu TF, Shen KH, Wang YH, Wu WR, Shiue YL, Huang HY, Hsu HP,
Chen YH, Chen LT, Li CF, Liao AC (2012). Rsf-1/HBXAP overexpression is
independent of gene amplification and is associated with poor outcome in
patients with urinary bladder urothelial carcinoma. Journal of Clinical
Pathology 65(9): 802-807.
36. Liao,
AC., LI, CF, Shen, KH, Huang, LC, Huang, HY, Wu, TF* (2011). Lactate
dehydrogenase-B subunit under-expression is associated with tumor progression
and independently predicts inferior disease-specific survival in urinary
bladder urothelial carcinoma. Pathology 43: 707-712.
37. Chuang
CH, Huang YW, Wu YT, Wu TF. (2011). Programmable Dielectrophoretic Chip for
Cell Manipulations. Japanese J. Applied Physics 50: 6GL11-1-11-6.
38. Liao
KA, Tsay YG, Huang LC, Huang HY, Li CF, Wu TF* (2011). Search for the
tumor-associated proteins of oral squamous cell carcinoma collected in Taiwan
using proteomics strategy. J. Proteome Res. 10: 2347-2358.
39. Chan
YY, Chang CS, Chien LH,Wu TF* (2010). Apoptotic effects of a high performance
liquid chromatography (HPLC) fraction of Antrodia camphorata mycelia are
mediated by down-regulation of the expressions of four tumor-related genes in
human non-small cell lung carcinoma A549 cell. J. Ethnopharmacology 127:
652-661.
40. Li
CF, Shen KH, Huang LC, Huang HY, Wang YH, Wu TF* (2010). Annexin-I
overexpression is associated with tumor progression and independently predicts
inferior disease-specific and metastasis-free survival in urinary bladder
urothelial carcinoma. Pathology 42: 43-49.
42. Wu
TF, Huang WC, Chen YC, Tsay YG, Chang CS. (2009). Proteomic investigation of
the impact of oxygen on the protein profiles of hyaluronic acid-producing
Streptococcus zooepidemicus. Proteomics 19(9): 4507-4518.
43. Cheng,
B.-C., Chang, C.-P., Tsay, Y.-G., Wu, T.-F., Hsu, C.-Y., and Lin, M.-T.*
(2008). Body Cooling Causes Normalization of Cardiac Protein Expression and
Function in A Rat Heatstroke Journal of Proteome Research 7, 4935-4945 (SCI).
44. Tsai,
W.-C., Pan, Z.-J., Hsiao, Y.-Y., Jeng, M.-F., Wu, T.-F., Chen W.-H., and Chen,
H.-H.* (2008). Interactions of B-class complex proteins involved in tepal
development in Phalaenopsis orchid. Plant and Cell Physiology 49, 814-824
(SCI).
45. Wu
TF, Hsu CY, Huang HS, Chou SP, and Wu H* (2007). Proteomic Analysis of
Pycnogenol Effects in RAW 264.7 Macrophage Reveals Induction of Cathepsin D
Expression and Enhancement of Phagocytosis. J Agric Food Chem 55, 9784-9791
(SCI).
46. Wu,
T.-F.*, Ku, W.-L., and Tsay, Y.-G. (2007). Proteome-based diagnostics and
prognosis of bladder transitional cell carcinoma. Expert review of proteomics
4, 639-647 (SCI).
47. Wu,
T.-F., Wu, H., Wang, Y.-W., Chang, T.-Y., Chan, S.-H., Lin, Y.-P., Liu, H.-S.,
and Chow, N.-H.* (2007). Prohibitin in the Pathogenesis of Transitional Cell
Bladder Cancer Anticancer research 27, 895-900 (SCI).
48. Wu,
H., Pan, C.-L., Yao, Y.-C., Chang, S.-S., Li, S.-L., Wu, T.-F.* (2006).
Proteomic analysis of the effect of Antrodia camphorata extract on human lung
cancer A549 cell. Proteomics 6, 826-835 (SCI).
49. Sheng,
K.-H., Yao, Y.-C , Chuang, S.-S., Wu, H., and Wu, T.-F.* (2006). Search for the
tumor-related proteins of transitional cell carcinoma in Taiwan by proteomic analysis.
Proteomics 6, 1058-1065 (SCI).
50. Wu,
T.-F., Wu, H., Chow, N.-H., Liao, C.-F., and Liu, H.-S.* (2005). Proteomic
analysis of bladder cancer cells reveals potential candidates of biomarkers in
bladder tumorigenesis. Cancer genomics and proteomics 2, 151-158 (Indexing in
Medline).
51. Chiang,
C.-L.*, Sung, C.-S., Wu, T.-F., Chen, C.-Y., and Hsu, C.-Y. (2005). Application
of superparamagnetic nanoparticles in purification of plasmid DNA from
bacterial cells. Journal of chromatography B 822, 54-60 (SCI).
52. Fang,
J.-D.*, Lin, H.-W., Lin, H.-L., Wu, H., and Wu, T.-F. (2003). The applications
of proteomics analysis by LC/MS/MS and in gel digestion method. Chemistry (The
Chinese Chem. Soc., Taipei) 61, 277-284.
53. Liu,
C.-Z.*, Wu, T.-F., Huang, T.-F., Wu, D.-H., and Lin G.-L. (2002). Trimucytin, a
collagen-like snake venom protein, activates platelets independent of I-domain
within alpha2 subunit of alpha2beta1 integrin. Thrombosis Research 105, 153-160
(SCI, IF=2.038).
54. Wu,
H.*, Shen, H.-W., Wu, T.-F., Brass, L. F., and Sung K.-C. (2002). Extracellular
Signal-Regulated Kinases and G protein-Coupled Receptors in Megakaryocytic
Human Erythroleukemia Cells: Selective Activation, Differential Regulation, and
Dissociation from Mitogenesis. J Pharmcol. Exp. Ther. 300, 339-345 (SCI,
IF=4.003).
55. Chen,
H.-H.*, Tso, D.-J., Yeh, W.-B., Cheng, H.-J., and Wu, T.-F. (2001). The
thymidylate synthase gene of Hz-1 virus: A gene captured from its lepidopteran
host. Insect Mol. Biol. 10, 495-503 (SCI, IF=2.787).
56. Wu,
T.-F.*, Chen, H.-H., and Wu, H. (2001). Functional characterization of Marek's
disease virus (MDV) origin-binding protein (OBP): analysis of its
origin-binding properties. Virus Genes 23, 227-239 (SCI, IF=1.362).
57. Wu,
T.-F.*, Hwang J. C., and Chen, H. H. (1999). Expression of Marek’s disease
virus (MDV) gene homologous to UL9 gene of herpes simplex virus type 1 (HSV-1)
by recombinant Baculovirus. Chia-Nan Bulletin 25, 165-177.
58. Liu,
W. H.*, Wu, T.-F., and Lo, C. K. (1997). Transformation of sitosterol to
testosterone by Mycobacterium sp. J. of the Chinese Agricultural Chemical
Society 35, 124-131 (國科會優良期刊).
59. Wu, T.-F.,
and Coussens, P. M.* (1996). Cloning and sequence analysis of a Marek’s disease
virus (MDV) gene homolog to herpes simplex virus type 1 (HSV-1) UL9. In :
Current Research on Marek‘s Disease, R. F. Silva, H. H. Cheng, P. M. Coussens,
L. F. Lee, and L.F. Velicer, eds. American Association of Avian Pathologist,
Kennett Square, PA. pp. 436-441.
60. Wu,
T.-F., Wei, S., Mekki, B., Southwick, R., and Coussens, P. M.* (1996). Cloning
and sequence analysis of a Marek’s disease virus origin binding protein (OBP)
reveals strict conservation of structural motifs among OBPs of divergent
alphaherpesviruses. Virus Genes 13, 143-157 (SCI, IF=1.362).